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Type I & Type II Water

Type I Water

Type 1 water systems are some of the most common types of systems we design and install. Type 1 water (also known as reagent grade or ultrapure water) is used for numerous types of critical laboratory applications.

Type I water is the grade required for critical laboratory applications such as HPLC mobile phase preparation, blanks and sample dilution in Gas Chromatography, HPLC, AA, ICP-MS and other advanced analytical techniques. Type I water is necessary for the preparation of buffers and culture media for mammalian cell culture and IVF. Type I water is specified in the production of reagents for molecular biology applications (DNA sequencing, PCR). And Type I water is also required in the preparation of solutions for electrophoresis and blotting.

Type II Water

Type II water is the grade used in general laboratory applications such as buffers, pH solutions and microbiological culture media preparation.  Type II water is the grade required as the feed water to Type I water systems, clinical analyzers, cell culture incubators and weatherometers.  Type II water is also the grade designated for the preparation of reagents for chemical

analysis or synthesis.

Using Type I grade water for Type II water applications is a common laboratory practice. Type I grade water decreases the risk of artifact generation during experimental procedures.

NCCLS Maximum Contaminant Levels in Type I Purified Water

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